Fig. 6.

F-actin clustering induced by actin-binding domain (ABD) fragments of the pathological FLNB mutants. HEK-293 and Rat2 cells expressing hemagglutinin (HA)-tagged FLNB residue 1–248, including ABD, were subjected to subcellular fractionation (A), and images were visualized by confocal immunofluorescence (B). HA-FLNB ABD was detected with anti-HA antibody. Extent of HA-FLNB ABD on immunoblot was determined by densitometry. Values are means ± SE from 3 independent transfections. *P < 0.05 (by Student's t-test). Arrow indicates position of a 37-kDa marker on each blot. Scale bar, 10 μm.