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. 2016 Jan 4;2(6):ENEURO.0072-15.2015. doi: 10.1523/ENEURO.0072-15.2015

Table 2.

Three-way repeated-measures ANOVA of reward expectancy ratings

Factor Mean square F Significance
Stage 0.233 F(1,17) = 0.320 0.579
Phase 13.498 F(1,17) = 18.622 0.000469
Stimulus 4.021 F(1,17) = 1.59 0.224
Stage × phase 0.274 F(1,17) = 0.437 0.518
Stage × stimulus 99.418 F(1,17) = 42.526 0.000005
Phase × Stimulus 0.341 F(1,17) = 0.627 0.439
Stage × phase × stimulus 11.724 F(1,17) = 8.951 0.008

There were no outliers in the data and the ratings were normally distributed, as assessed by inspection of a boxplot and Shapiro–Wilk’s test of normality (all p > 0.05). In this analysis, the assumption of sphericity for all three main factors (stage, phase, and stimulus) and their two- and three-way interactions was automatically met, because all these factors had only two levels. As shown above, there was a statistically significant three-way interaction between stage, phase, and stimulus (F(1,17) = 8,961, p = 0.008). Bonferroni corrected post hoc tests comparing the difference in reward expectancy ratings between CS+ and CS− at each stage showed a significantly higher reward expectancy rating of color A compared to color B during late acquisition (p = 0.007). In the reversal stage, a significantly higher differential reward expectancy rating of the new CS+ versus the new CS− was observed (p = 0.017).