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. 2016 Jan 4;6:18685. doi: 10.1038/srep18685

Figure 5. Plastic expression of TERT in murine macrophages.

Figure 5

RAW264.7 cells were treated with LPS (1 μg/mL) for 24 h to polarize M1 macrophage phenotype, while treatment with IL-4 (15 ng/mL) for 24 h induced M2 macrophage phenotype. One population into another was transformed by culturing M1 macrophages with IL-4 and M2 macrophages with LPS, respectively. (a) The mRNA levels of M1 macrophage markers (TNF-α, IL-1β, CCL2 and NOS2) and M2 macrophage markers (Arg-1, IL-10, Mrc2 and CD163) were analyzed by real-time PCR. (b) The plastic expression of TERT in murine macrophage polarization was determined by real-time PCR and western blot. The results are shown as relative expression against control expression without treatment. Data shown are the mean ± SD from 3 independent experiments. *P < 0.05, **P < 0.01 vs control. (c) The expression of TERT in RAW264.7 macrophages polarization was analyzed by immunofluorescence (IF) assay. Representative views from each group were presented (original magnification, ×20). (d) RAW264.7 cells were treated with IFN-γ (10 ng/mL) for 24 h alone or in combination with LPS. The production of TERT was determined by real-time PCR and western blot. The results are shown as relative expression against control expression without treatment. Data shown are the mean ± SD from 3 independent experiments. *P < 0.05, **P < 0.01 vs control.