Table 1.
Oligonucleotides for SMIM1 genotyping
| Name | Allele specificity | Direction | Sequence (5'–3') | Amplicon size, bp |
|---|---|---|---|---|
| PCR-SSP | ||||
| SMIMlwt-F | wild type | sense | GCAGCAGGGACGGAGTCA | 216 |
| SMIM1del-F | 64-80del | sense | GCAGCAGGGACGGAGTCC | 199 |
| SMIM1-R | SMIM1 | antisense | CCAAAGGCTGCGGTTTGCTG | |
| Rs1175550-F1a | Major A | sense | TCAGGGGCTGCAGCCTAGA | 160 |
| Rs1175550-F1g | minor G | sense | TCAGGGGCTGCAGCCTAGG | 160 |
| Rs1175550-R1 | SMIM1 | antisense | CTCAGGCCAAGCCCTGACC | |
| TaqMan PCR | ||||
| SMIM1-F1 | SMIM1 | sense | GCCACGTCCACTATAGTAGG | 142/125 |
| SMIM1-R1 | SMIM1 | antisense | GGAGACACCAGCCTGCTATG | |
| SMIM1wt-P | wild type | sense | VIC-CTAGGGGCTGTGTC-MGB* | |
| SMIM1del-P | 64-80del | sense | FAM-ACGGAGTCCAGCACAG-MGB* |
*
TaqMan probe modifications: FAM, VIC, minor groove binding (MGB).