Figure 2. Clonogenic assay.

Figure 2 shows the cell surviving fraction observed after drug treatment with LP-CC6 ranging from 0% (MCF-7, A2780 cell lines) to 58% (A375 cell line) and, for LP-CC24, from 7% (HuH-7) to 71.8% (A375) while the surviving fraction of tumour cells in the presence of CisPt alone at the same concentration was approximately 60%. The plating efficiency and the surviving fraction for each cell line after each treatment were calculated according to the method proposed by Franken et al.³⁵ and averaged approximately 80% for all cell lines. Survival was calculated compared with non-treated samples using an average of three determinations of the same dose rate of cells (±SE). The average values with a standard deviation were determined from three independent experiments using different LP-CC6, LP-CC24 and CisPt preparations.