Table 2. Cytotoxicity in cell culture lines induced by different treatments.
| CELL LINE | TREATMENTS | LDH Estimates |
P-values (***) |
||||
|---|---|---|---|---|---|---|---|
| Mean (*) | Var (**) | LP | LP-CC6 | LP-CC24 | CisPt | ||
| DU145 | LP | 18.2 | 0.367 | 1.000 | 1.000 | – | 1.000 |
| LP-CC6 | 54.2 | 0.108 | 0.000 | – | 0.000 | 0.000 | |
| LP-CC24 | 36.0 | 0.369 | – | 1.000 | 0.000 | 0.003 | |
| CisPt | 32.0 | 0.056 | 0.997 | 1.000 | 0.000 | – | |
| A2780 | LP | 4.0 | 0.095 | – | 1.000 | 1.000 | 1.000 |
| LP-CC6 | 85.0 | 0.067 | 0.000 | – | 0.000 | 0.000 | |
| LP-CC24 | 54.0 | 0.032 | 0.000 | 1.000 | – | 0.000 | |
| CisPt | 34.0 | 0.805 | 0.000 | 1.000 | 1.000 | – | |
| MCF-7 | LP | 22.0 | 0.036 | – | 1.000 | 1.000 | 0.990 |
| LP-CC6 | 73.0 | 0.042 | 0.000 | 0.002 | – | 0.000 | |
| LP-CC24 | 68.0 | 0.334 | 0.000 | – | 0.998 | 0.000 | |
| CisPt | 25.0 | 0.771 | 0.010 | 1.000 | 1.000 | – | |
| A375 | LP | 4.5 | 0.032 | – | 1.000 | 1.000 | 1.000 |
| LP-CC6 | 38.0 | 0.411 | 0.000 | – | 0.002 | 0.004 | |
| LP-CC24 | 33.6 | 0.005 | 0.000 | 0.998 | – | 0.972 | |
| CisPt | 35.0 | 0.543 | 0.000 | 0.996 | 0.028 | – | |
| HuH-7 | LP | 2.0 | 0.705 | – | 1.000 | 0.999 | 1.000 |
| LP-CC6 | 69.0 | 0.060 | 0.000 | – | 0.000 | 0.000 | |
| LP-CC24 | 32.0 | 0.532 | 0.000 | 1.000 | 0.000 | – | |
| CisPt | 10.0 | 0.155 | 0.001 | 1.000 | – | 1.000 | |
| PaTu- 8902 | LP | 6.5 | 0.008 | – | 1.000 | 1.000 | 1.000 |
| LP-CC6 | 34.0 | 0.512 | 0.000 | – | 1.000 | 1.000 | |
| LP-CC24 | 46.0 | 0.478 | 0.000 | 0.000 | – | 0.996 | |
| CisPt | 49.0 | 0.538 | 0.000 | 0.000 | 0.004 | – | |
| MCF-10A | LP | 22.0 | 0.402 | – | 0.992 | 0.999 | 1.000 |
| LP-CC6 | 24.0 | 0.307 | 0.008 | – | 0.995 | 1.000 | |
| LP-CC24 | 26.0 | 0.245 | 0.001 | 0.005 | – | 0.999 | |
| CisPt | 30.0 | 0.219 | 0.000 | 0.000 | 0.001 | – | |
Table 2 shows the cytotoxic activity exerted by synthetic LP-CC6, LP-CC24 and CisPt alone and illustrates the mean level estimates of LDH based on the following triplicate treatments of cell lines. The hexapeptide showed more significant activity, except in PaTu-8902 cells, with a noticeable peak in ovarian (85.0%), breast (73.0%) and hepatocellular carcinoma cells (69.0%).
The data were obtained from 3 independent observations (the bars indicate the s.d.). The mean level estimates of LDH (cytotoxic effect) in the cell lines treated with LP, LP-CC6, LP-CC24 and CisPt;(N = 3).
*Sample mean and **unbiased estimate of the population variance. ***based on Welch’s test for the null hypothesis that the mean level of uptake for the treatment in the row is less or equal to the mean level of uptake for the treatment in the column, against the alternative that the former is greater than the latter.