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. 2015 Nov 18;29(1):1–15. doi: 10.5713/ajas.16.0001R

Figure 2.

Figure 2

Inhibition of TNF-α-induced upregulation of cheme rin and CMKLR1 gene expression in differentiated bovine adi pocytes. Cells were incubated with serum-free DMEM/HAMF 12 for 2 h before treatment. Cells were pretreated with vehic le (DMSO) or MAPKs inhibitors (PD98059, SP600125, or S B203580) for 1 h, and then stimulated with TNF-α for 3 h (A, B). After treatment for 3 h, the expression levels of che merin and CMKLR1 gene were analyzed by qRT-PCR. Gene expression was normalized with ALAS1 and represented as t he mean±SEM (n = 6). abc Significant differences (p<0.05) between values for each treatment concentration are denoted with letters. CMKLR1, chemokine receptor-like 1; TNF-α, tumor necrosis factor-α; MAPK, mitogen-activated protein kinase; ALAS1, aminolevulinate synthase 1.