Figure 2. TLR signalling properties of the commensal bacteria. NF-κB activation was measured using a luminescence reporter in HEK293 cell lines expressing TLR2, TLR2/6, TLR5 and TLR4 after incubation with the bacteria (bacterium: cell ratio, 10:1).
NF-κB activation is expressed as percentage of the positive control. Error bars represent SEM, n = 6, *indicates p < 0.05, **p < 0.01, ***p < 0.001 compared to the control.