Figure 5.

Analysis of B cells and CD4⁺T cells in mice with acute graft-versus-host disease (GVHD), in whom splenocytes were treated with IL-1 receptor antagonist (IL-1Ra): (a) to elucidate the in vivo mechanism of action of the blockade of IL-1 signaling in amurine model of acute GVHD, we analyzed the proportion of Th1, Th2, and Th17 and (b) shows T_reg cells among ex vivo splenocytes from each group by means of flow cytometry. (c) The share of the B-cell subset was analyzed using flow cytometry. B220⁺ B cells included IgM^highIgD^low (immature B cells) and IgM^lowIgD^low (mature B cells). (d) shows The level of IgG in serum. ^∗ P < 0.05, ^∗∗ P < 0.01. Data are representative of 2 independent experiments.