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. Author manuscript; available in PMC: 2016 Jan 4.
Published in final edited form as: J Cell Biochem. 2015 Aug;116(8):1540–1552. doi: 10.1002/jcb.25095

Figure 8.

Figure 8

RNA-Seq expression data was validated at the protein level. (A) HPM3 cells were exposed to asbestos (1(15 × 106) or 5 (75 × 106) µg/cm2) for 8 h. Medium was collected and concentrated as described in the method section. Concentrated medium was analyzed for IL-1β (ELISA assay), IL-6 or IL-8 (Western blot analysis). N=3 samples/group. *p≤0.05 as compared to untreated control. (B) Validation of some highly expressing genes by qRT-PCR.