Figure 3. Transduction with CD40L lentiviral expression vectors induces DCs to produce Th1-skewing and proinflammatory cytokines.
DCs were transduced with lentiviral vectors that contained or lacked Vpx and after 72 hours cytokine production was measured in the culture supernatant. (a) The quantification of IL-12 is shown. (b) The quantification of TNF-α is shown. The results are the average data from three donors assayed in triplicate with error bars indicating mean +/− SD. *p<0.05, **p<0.01, and ***p<0.001 as determined by paired two-tailed t test.