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. Author manuscript; available in PMC: 2016 Jan 4.
Published in final edited form as: Gene Ther. 2015 Jan 8;22(3):227–236. doi: 10.1038/gt.2014.117

Figure 4. DCs transduced with CD40L lentiviral expression vectors induce T cells to proliferate.

Figure 4

DCs were transduced with Control LV or CD40L vectors that contained or lacked Vpx and after 48 hours, cocultured with CFSE-labeled leukocytes. Seven days later, the cells were stained with fluorescent anti-CD3, CD4, and CD8 mAbs and analyzed by flow cytometry. As controls, CFSE-labeled leukocytes were cultured alone (Mock) or with uninfected DCs. (a) CFSE staining of the CD3+/CD8+ T cells is shown. (b) CSFE staining of the CD3+/CD4+ T cells is shown. The mock and uninfected controls are above and cocultures of DCs transduced with Control LV or CD40L vectors are below. (c) CFSE staining of the CD3+/CD8+ and CD3/CD4+ T cells of three donors is shown. The average number of CFSE-low+ cells is shown with error bars indicating mean +/− SD. *p<0.05, **p<0.01, and ***p<0.001 as determined by paired two-tailed t test.