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. Author manuscript; available in PMC: 2016 Jan 4.
Published in final edited form as: Mol Pharmacol. 2008 Jul 24;74(4):1022–1032. doi: 10.1124/mol.108.046250

Fig. 7.

Fig. 7

PKC is not upstream of p38 MAPK in response to TNF. A and B, A549 cells (200 × 103) were seeded in 60-mm dishes; 48 h later, they were preincubated with bisindolylamide I (2 μM) or Go6976 (3 μM) (A) or rottlerin (10 μM) for 1 h before stimulation with TNF (50 ng/ml, 10 min) (B). C, A549 cells (65 × 103) were seeded and, 24 h later, transfected with negative control (Scr) or novel PKC siRNA. After 48 to 60 h, media were changed and cells were incubated for 1 to 2 h and stimulated with TNF (3 nM, 10 min). Total protein samples were prepared and analyzed for phospho-p38 MAPK and p38 MAPK as described under Materials and Methods. (*, P < 0.05 versus unstimulated, n = 5).