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. 2015 Dec 21;2016:8539057. doi: 10.1155/2016/8539057

Figure 2.

Figure 2

Phenotypic characterisation by flow cytometry of the adipose SVF cells and PBMCs in lean and obese pigs. (a) Gating strategy of the porcine adipose SVF cells from an obese pig. T-cells were defined as CD45+CD3+. Within the T-cells, CD4+, CD8+, and CD4+CD8+ were characterised based on positivity for CD4 and CD8. Macrophages were defined as CD45+CD3−CD203A+, and the M1 and M2 macrophages were determined based on positivity for CD11R3 and CD163, respectively. B-cells were CD45+CD3−CD203A− and CD21+ whereas NK-cells were CD45+CD3−CD203A− and NKp46+. (b–d) Infiltrating leukocyte populations in the adipose tissue within the CD45+ population from lean (grey columns) and obese (white columns) pigs. (b) Frequency of the total macrophages (CD203A+), classically activated M1 macrophages (CD203+CD11R3+), and alternatively activated M2 macrophages (CD203+CD163+). (c) Frequency of natural killer cells (NKp46+) and B-cells (CD21+). (d) Frequency of the T-helper (CD3+CD4+), T-cytotoxic (CD3+CD8+), and double positive T-cells (CD3+CD4+CD8+) within the T-cells. (e–g) PBMCs subpopulation frequencies. (e) Lymphocytes and monocytes. (f) Natural killer cells (CD3−NKp46+) and B-cells (CD3−CD21+) populations frequency. (g) Frequency of the T-helper (CD3+CD4+), T-cytotoxic (CD3+CD8+), and double positive T-cells (CD3+CD4+CD8+) within the T-cells. Data are mean ± SD. ∗ p < 0.05.