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. 2015 Dec 21;2016:6301712. doi: 10.1155/2016/6301712

Figure 2.

Figure 2

225THC is nontoxic and inhibits inflammatory cytokine responses in stimulated primary rat microglial cultures. Effects of 225THC on primary rat microglial cells. (a) Toxicity assay of 225THC on primary cultured rat microglia. Data shown from three separate experiments represent the mean number of live primary microglia (as assessed by live/dead assay) present per field of view following incubation for 24 h with different concentrations of 225THC (0, 1, 5, 10, 50, and 100 μM) for 24 h with or without coactivation of the microglia with LPS (2 μg/mL). (b) ELISA assessment of secreted cytokines in LPS (2 μg/mL) activated primary rat microglia cultures treated with 225THC at the concentrations indicated. Statistical analysis was performed by a two-way ANOVA with Bonferroni post hoc analysis; ∗ indicates p < 0.05, ∗∗ p < 0.01; when compared with basla or as indicated.