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. 2015 Dec 21;2016:9581350. doi: 10.1155/2016/9581350

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Copyright © 2016 Sun-A Ock et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Xenogeneic human mixed lymphocyte reaction (MLR) test to confirm immunomodulation capacity of mesenchymal stromal/stem cells (MSCs). Tissue-specific MSCs were isolated from 3 pigs. Peripheral blood mononuclear cells (PBMCs) were cocultured with MSCs in 2 ratios (PBMCs : MSCs of 1 : 1 and 10 : 1). (A) and (B) show the result of the MLR test for each MSC type, by MSC source, based on the average value obtained from the 3 pigs. (C) Cell states at 72 h after cell seeding on 96-well plates. Scale bars = 500 μm.