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. 2015 Dec 21;2016:2524092. doi: 10.1155/2016/2524092

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Copyright © 2016 Hui Liu et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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RSV enhances CM differentiation partially through inhibition of WNT signal pathway. (a) An outline describing the administrations of pathway modulators during CM differentiation. (b) TOP Flash assay of human iPSCs treated with different concentrations of RSV in suspension culture condition at day 4. (c) From day 0 to day 5, TOP Flash assays were performed, respectively, after 50 μM RSV incubation. (d, e). The effects of different concentrations of DKK1, WNT inhibitor (d) and WNT3A, WNT activator (e) on the ratio of beating EBs were evaluated on day 24 (n = 100). Data represent the mean ± s.d. of three biological replicates. ^∗ P < 0.05 compared with control; ^# P < 0.05 compared with RSV alone.