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. 2015 Dec 21;10(12):e0145280. doi: 10.1371/journal.pone.0145280

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Fig 2 — A. REST KD and NT hESCs (H1 and H9) were evaluated for BrdU incorporation by FACS analysis for TRA-1-81 and BrdU double positive cells. B. Percentage of TRA-1-81and BrdU positive cells were not statistically significantly different in REST KD compared to control NT cells. Error bars represent standard error of three independent experiments (performed at p52, p54 and p60 for H9 cells, and at p47, p49, and p50 for H1 cells). C. REST KD and NT hESCs (H1 and H9) were evaluated for the percentage of Annexin V and DAPI positive cells by FACS analysis. D. FACS analysis for Annexin V and DAPI staining demonstrated that REST KD hESCs have statistically significant improvements in survival as demonstrated by reduced levels of apoptotic cells (p<0.032 in H9 and p<0.002 in H1) compared to control NT hESCs. Error bars represent standard error of three independent experiments (performed at p55, p57 and p59 for H9 cells, and at p55, p56, and p57 for H1 cells) and asterisks denote a p value < .05 using Student’s t-test analysis.