Table 1. Physiological parameters of E. coli cultures (in M9 medium supplemented with 2.5 g/L of glucose) as a function of the concentration of extracellular AgNO3.
The value μt = 3h corresponds to the specific growth rate calculated between 2.5 and 3.5 h of culture. The μexpo value is the specific growth rate over the entire exponential growth phase (start and end specified in brackets) after the beginning of growth. The mean ± SD values of three independent experiments are given, except at a concentration of 6.5 μM AgNO3 for which the values of all three experiments are detailed.
| [AgNO3] in the medium (μM) | Lag time (h) | μt = 3h (h-1) | Number of cultivable cells at 3 h (CFU/mL/ODU) | μexpo (h-1) | Maximum absorbance (at 595 nm) |
|---|---|---|---|---|---|
| 0 (control) | 0.5 ± 0.0 | 0.62 ± 0.01 (from 2.5 to 3.5 h) | 5.2 ± 1.3 x 108 | 0.63 ± 0.01 (from 0.5 to 5.5 h) | 3.31 ± 0.12 |
| 5.0 | 0.5 ± 0.0 | 0.63 ± 0.03 (from 2.5 to 3.5 h) | 4.3 ± 1.2 x 108 | 0.59 ± 0.03 (from 0.5 to 5.5 h) | 3.19 ± 0.11 |
| 6.5 | 9 | 0 | 2.4 x 108 | 0.54 (from 10.0 to 14.5 h) | 3.13 |
| 6.5 | 17 | 0 | 2.3 x 108 | 0.44 (from 18.0 to 24.5 h) | 3.36 |
| 6.5 | > 24 | 0 | 4.3 x 107 | 0 | 0.11 |
| 8.5 | > 24 | 0.0 ± 0.0 | 1.1 ± 0.2 x 107 | 0.0 ± 0.0 | 0.10 ± 0.01 |
| 100 a | — | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | — |
a: values from [15].