Table 1. Colony forming units of E. coli transformed with golden gate products and grown on ampicillin plates for each of the 3D printed fluidics.

Device	Assembly Reaction	No. of Samples	Mean CFU (x 105)	S a (x 105)
Tube control
	No enzyme	4	0.00	0.00
	Enzyme & DNA	7	1.60	0.80
Form 1+ co-laminar
	No enzyme	1	0.00	0.00
	Off device mix b	6	1.42	0.83
	On device mix c	5	1.30	1.20
Form 1+ micromixer
	No enzyme	1	0.00	0.00
	Off device mix b	5	1.74	1.42
	On device mix c	7	1.71	1.54
Shapeways co-laminar
	No enzyme	1	0.00	0.00
	Off device mix b	5	1.79	1.67
	On device mix c	5	2.53	0.52

^a. Standard deviation.

^b. Golden Gate enzymes and DNA mixed off device and incubated on device.

^c. Golden Gate enzymes and DNA mixed on device and incubated on device.