High GFP-positive rates in granulocytes and monocytes at 1–2 months after RIC HSC transplantation. (a) At 2 weeks after transplantation in both animals, we observed ~2% of GFP-positive rates (%GFP) in RBCs, but undetectable in granulocytes, lymphocytes, monocytes or platelets. %GFP in RBCs gradually decreased and was undetectable by 3 months. Interestingly, %GFP in granulocytes and monocytes were unexpectedly high (80–99%) starting at 1–2 months after transplantation, and declined to undetectable levels by 3–4 months. (b) In lineage marker specific analysis (CD3, CD20, CD33, CD41a and RBC-specific antibodies), similar patterns were observed.