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. 2015 Dec 18;16:149. doi: 10.1186/s12931-015-0309-0

Table 1.

Reverse transcription and real time PCR primer

Stage Temp Time
Reverse transcription hold 37 °C 120 min
hold 25 °C 10 min
PCR hold 50 °C 2 min
denature 95 °C 15 s
anneal/extend 60 °C 1 min
hold cycle (40 cycles) 95 °C 10 min
Primer Sense 5′ AGATGAGTGACCGAGGAG
Antisense 3′ TATACACTGGGAGTCTTTCT