Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Dec 15;29(24):2563–2575. doi: 10.1101/gad.272633.115

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Fei et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Figure 4. — Prenucleosomes can be converted into canonical nucleosomes by ACF. (A) ACF-dependent assembly of poly-prenucleosomes to polynucleosomes. Mono-prenucleosomes (prepared by salt dialysis with 80-bp genomic DNA containing two 5-nt overhangs) were ligated in a sequential head-to-tail fashion with free DNA (90 bp with two 5-nt overhangs) to give poly-prenucleosomes, as indicated in the diagram. The resulting poly-prenucleosomes were assembled into polynucleosomes with ACF and ATP. The formation of canonical nucleosomes was verified by MNase digestion of the polynucleosomes into core particles, which contain ∼147 bp of DNA. (B) ACF-dependent assembly of mono-prenucleosomes to canonical nucleosomes. Mono-prenucleosomes (prepared by NAP1-mediated histone deposition with the central 80 bp of 601 DNA containing two 5-nt overhangs) were ligated to two free 80-bp DNA fragments (each containing a single 5-nt overhang) to give mono-prenucleosomes that are flanked by 85-bp DNA extensions, as illustrated in the diagram. The resulting mono-prenucleosomes were assembled into nucleosomes by ACF. The formation of canonical nucleosomes was assessed by MNase digestion into core particles that contain ∼147 bp of DNA. The 80-bp and 165-bp DNA fragments are incomplete ligation products.