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. Author manuscript; available in PMC: 2016 Jan 4.
Published in final edited form as: J Am Chem Soc. 2015 Nov 9;137(45):14260–14263. doi: 10.1021/jacs.5b10128

Figure 2.

Figure 2

Minimal proton linkage accompanies dUMP binding to TSase. Modified general model was used to fit ITC experiments in multiple buffers with different enthalpies of ionization (ΔH° b). Buffer color key in (C) applies to all panels. In panels A, B, and D, sites 1 (2) are represented by filled (open) bars/symbols. Slopes of lines in panel D give number of protons (n) linked to binding. Site 1 (•) fits to n of −0.09 ± 0.02 protons and ΔHiof −4.8 ± 0.17 kcal/mol. Site 2 (∘) fits to n of −0.06 ± 0.03 protons and ΔHiof −4.5 ± 0.19 kcal/mol. Example fits are given in Figure S5, with values in Table S3.