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. Author manuscript; available in PMC: 2016 Jan 4.
Published in final edited form as: J Am Chem Soc. 2015 Nov 9;137(45):14260–14263. doi: 10.1021/jacs.5b10128

Table 1.

Relative binding constants for TSase 5F-dUMP-CH2H4Fol di-ligand binding from NMR titration.

Residue ρ a
Gln33 0.88 ± 0.067
Ile129 0.90 ± 0.12
Asn134 0.57 ± 0.080
Unassigned Trp Indole 0.77 ± 0.14
Global fit 0.65 ± 0.075
a

Ratio of intrinsic association constants (K2/K1)