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. Author manuscript; available in PMC: 2016 Jan 4.
Published in final edited form as: J Immunol. 2009 May 15;182(10):6307–6315. doi: 10.4049/jimmunol.0802454

FIGURE 3.

FIGURE 3

Kinetic analysis of IFN-γ-inducible gene expression in mouse TS cells and giant cells. RNA was isolated from NIH-3T3, TS cells, and giant cells exposed to 500 U/ml IFN-γ for 0, 3, 6, and 24 h and subjected to SYBR Green-based quantitative RT-PCR using primers specific for IRF-1, GBP, and GAPDH, as described in Fig. 2. The data are the average of three independent experiments and are represented as the ratio of the relative mRNA expression of each gene (i.e., IRF-1) vs GAPDH. Unpaired Student’s t test was used to compare the relative levels of IFN-γ-inducible gene expression in NIH-3T3 cells vs TS and giant TBCs that were exposed to IFN-γ for the same duration.