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. 2015 Dec 14;10(12):e0144851. doi: 10.1371/journal.pone.0144851

Fig 2. The porcine Prdx6 gene promoter was analyzed by computational analyses together with luciferase reporter system.

Fig 2

(A) Conserved sequences of transcription factor binding sites of Prdx6 promoter. The aligned region is conserved among pig, human and bovine. (B) Six promoter constructs were transfected into C2C12, 3T3-L1 and PK15 cell lines, and assayed for luciferase activity. The pGL3-Control and pGL3-Basic were the positive and negative control respectively. Green, orange, blue, red and bright blue dots on the left panel represented the C/EBPβ, MyoD, CREB, Sp1 and HSF binding sites predicted on TFsearch or TESS websites, respectively. Data are expressed as means ± SD of three replicates. (C) Four further deletions (D5.1, D5.2, D7, and D8) were transfected into C2C12 cells and assayed for luciferase activity.