Skip to main content
. 2015 Dec 14;10(12):e0144851. doi: 10.1371/journal.pone.0144851

Fig 3. C/EBPβ and CREB could up-regulate the Prdx6 expression.

Fig 3

(A) The Prdx6 deletions were co-transfected with relative overexpressed vector or pcDNA3.1 empty vector. The promoter activity was measured and the results were expressed as means± SD of three replicates. (B) Schematic structure of site-direct mutants of Prdx6 promoter linked to pGL3-Basic vector. (C) Wild-types and mutants of the Prdx6 deletions were transfected into C2C12 cells, and luciferase activity was detected and the results were expressed as means± SD of three replicates. (D) The Prdx6 mRNA expression level was detected by RT-PCR after overexpression of each transcription factor into PK15 cells, data were showed as means± SD of three replicates. (E-G) The transfection efficiency of C/EBPβ, CREB or HSF1 overexpression vector and their effect on target Prdx6 protein expression level were determined by western blot. (H-I) The interference efficiency of Knockdown of C/EBPβ or CREB and their effect on target Prdx6 protein expression level were determined by western blot. Quantification results of western blot represented by ratio of C/EBPβ, CREB, HSF1 or Prdx6 to β-actin protein expression level (Image J software).