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. 2015 Dec 11;10(12):e0144251. doi: 10.1371/journal.pone.0144251

Fig 4. The shift in the pKa of F-neo in the presence of mature duplex miRNA.

Fig 4

A) The absorbance spectra of 1 μM F-neo in 10 mM NaPO4, 25 mM KCl, and 0.05 mM EDTA pH 7.2, 6.4, and 5.0. B) The absorbance at 490 nm of F-neo as a function of pH in the absence (black) and presence (blue) of the mature duplex hsa-miR 504.