Table 4. Compound classes with large deviations from the mean binding of the compound library.

	hsa-miR 142	hsa-miR 335	hsa-miR 504	Pre-hsa-miR 504
σ for all NeoXX compounds	21%	20%	23%	20%
Compounds σ averaged over all variations at position 1 and no amino acids at position 2. a
NeoX*	-0.43	0.80	0.26	-0.09
Compounds σ averaged over all variations at position 2 with constant amino acids at position 1. a
NeoRX	0.64	0.75	0.63	1.06
NeoDX	-0.92	-0.94	-1.05	-1.14
NeoWX	-0.43	-0.54	-0.38	-0.20
Compounds σ averaged over all variations at position 1 with constant amino acids at position 2. a
NeoXD	-1.22	-1.38	-1.52	-0.70
NeoXW	-0.67	-0.59	-0.36	-0.12
NeoXS	1.21	0.21	1.20	0.90
NeoXT	0.95	0.58	0.17	0.45
NeoXY	0.61	0.78	0.26	0.39
NeoXβA	-0.57	0.55	-0.42	-0.41
NeoXH	-0.55	-0.25	0.80	-0.04
NeoXR	0.13	0.74	0.36	-0.34
NeoXL	-0.27	-1.05	0.00	-0.71
NeoXF	0.32	-0.88	-0.17	-0.41
NeoXC	0.11	0.40	-0.66	-0.46
NeoXV	0.17	0.30	0.28	0.78

^aThe average binding and standard deviation from the mean (σ) was taken for all compounds in the library. The σ for the binding was determined for each compound. The average σ for all compounds with the indicated amino acid was determined for all compounds variations at position X.

*X indicates the variable amino acids A, R, N, D, H, L, F, P, S, T, Y, V, C, W. The amino acid K is included for in X for NeoX, NeoXS, NeoXT, NeoXY, and NeoXV. For data on percent binding and σ for all compounds, please refer to S1–S9 Tables.