Figure 7.
Neutrophil-mediated delivery of antibiotic to infected lungs. (A) Number of leukocytes and neutrophils, (B) proteins, (C) IL-6, and (D) TNF-α in BALF after iv injection of 5% glucose and Cefo-A BSA NPs (25 mg/kg) in mice 12 h after intratracheal (it) LPS challenge (8 mg/kg). Samples were collected 12 h later. (E) Total colony forming units of P. aeruginosa in BALF after iv injection of 5% glucose, vehicle of Cefo-A solution, Cefo-A solution (25 mg/kg) and Cefo-A BSA NPs (25 mg/kg) in mice 12 h after P. aeruginosa infection (1 × 106 cfu/mL, 40 µL per mouse). Samples were collected 12 h later. All data represent mean ± SD (3–4 mice per group). Statistics were performed by a two-sample Student's t test (*P < 0.05).