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Fast hydrazone formation of 2-APBA. a) Illustration of peptide (CGAB1) labeling via hydrazone conjugation; the side chain structure of AB1 is highlighted in bold. b) Kinetic profile of peptide labeling with Phzn. c) Illustration of VHP35 labeling via the hydrazone chemistry. d) Kinetic characterization of VHP35 labeling with Phzn. e) Inhibition of VHP35 labeling by abundant biomolecules; the results are tabulated by showing % of labelled proteins.
