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CD spectra of designed DM peptides and Ocr99. All DM peptides were random coils in borate buffer (A). A transition from random coil to coiled coil was observed in (NH4)2SO4 buffer for DM1–SS–DM1, DM2–SS–DM2 (B) and Ocr99 (C). All measurements were taken in 50 mM borate buffer pH 9.0 containing 1.5 M NH4(SO4)2 at 20 °C. [DM peptides] = 50 μM, [Ocr99] = 25 μM. Data represent the average of four scans.
