Table 1. Summary of inhibitory action of DMs and controls.
| Inhibitor | Residue / Charge | ATPase50 [a] (μM) / (Relative activity) | scDNA50[b] (μM) |
|---|---|---|---|
| DM1* | 29 / –11 | 95.2±7.0 (512) | 172.6±3.9 |
| DM1-SS-DM1 | 60 / –22 | 22.5±0.2 (121) | 38.0±0.9 |
| DM2-SS-DM2 | 62 / –22 | 36.1±0.2 (194) | 63.9±0.7 |
| DMscr-SS-DMscr | 62 / –22 | 182±13.4 (978) | 612.0±19.5 |
| DMc-SS-DMc | 62 / –22 | 237.3±13.4 (1276) | 245.5±14.7 |
| Ocr99 | 99 / –18 | 0.186±0.004 (1) | 0.172±0.011 |
| T7 Ocr | 14 / –7 | not active | not active |
| R46 ArdA | 14 / –2 | not active | not active |
[a]
Half maximal ATPase activity inhibition (ATPase50) represents the peptide concentration at which 50% of ATPase activity of the enzyme is inhibited. ATPase50 values were calculated by fitting a straight line to the average relative rate vs. peptide concentration data (n≥3).
[b]
Peptide concentration at which 50% of total scDNA. scDNA50 was determined by fitting %scDNA vs. peptide concentration obtained from densitometric analysis of agarose gels (n≥3). The data is represented as mean±SEM.
*
No N-terminal cysteine residue.