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. 2015 Nov 2;35(1):13–25. doi: 10.3892/or.2015.4371

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Copyright: © Baharuddin et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Sorting of double-positive (CD166⁺/EpCAM⁺) and double-negative (CD166⁻/EpCAM⁻) subpopulations in NSCLC cell lines (A549 and H2170). Both NSCLC cell lines were analysed for subpopulations using CD166-PE and EpCAM-FITC and sorted by flow cytometry. A549 cells showed 4.5% (Q2) expression of double-positive and 1.7% (Q3) expression of double-negative subpopulations (A), while H2170 presented with 3.1% (Q2) double-positive and 0.3% (Q3) double-negative subpopulations (B).