Figure 7.

Curcumin enhances the cisplatin-induced metastatic inhibition of the CSC subpopulation (CD166⁺/EpCAM⁺) in the NSCLC cell lines. Representative photomicrographs and analysis of wound closure are presented for sorted NSCLC cell lines after 48 h of curcumin treatment either alone or in combination with cisplatin using the IC₅₀ values (A and C). Higher migratory potential was noted in the double-positive (CD166⁺/EpCAM⁺) CSC subpopulation as compared to the unsorted of both NSCLC cell lines (B and D). Combination of both curcumin and cisplatin markedly inhibited the migration of CSCs in both the A549 and H2170 cells, suggesting better efficacies of the combination treatment for inhibiting the highly migratory CSC subpopulation (B and D). ^*P<0.01, ^**P<0.001; t-test.