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mRNA and protein levels of Cks1/Cks2 after transfection. (A) Cks1 and Cks2 mRNA levels after Cks1/Cks2 siRNA transfection by real-time PCR. (B) Cks1 and Cks2 protein levels examined by western blot analysis at 48 h after siRNA transfection. **P<0.01. The Cks1/Cks2 siRNA transfection procedure resulted in significant decrease in the transcriptional and protein expression levels of Cks1 and Cks2, indicating excellent knockdown efficiency.
