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. 2016 Jan 4;12(1):e1005366. doi: 10.1371/journal.ppat.1005366

Fig 3. In vivo knockdown of Brd4 or MEK1 reduces papilloma growth in rabbits.

Fig 3

(A) Schematic view of the recombinant CRPV genome carrying the shRNA expression cassette (pLAII-CRPVshRNA). (B) Brd4 silencing by three different siRNAs 48h after transfection into AVS cells [27]. siControl: Allstar; Negative Control siRNA; Mock: HiPerFect reagent. (C) Brd4 silencing in 293T cells transduced with lentiviral vectors carrying different shRNAs (pLVTHMshBrd4-1/-2); negative control pLVTHMshLuciferase. Alpha-tubulin was used as loading control. Molecular weight is indicated on the left. (D) Rabbit skin infected with pLAII-CRPVshRNA directed against Brd4 and with pLAII-CRPVshLuciferase. Six skin sites were infected with each construct per rabbit. The injection sites are marked with tattoo ink. Images were taken 6 months post infection. (E) MEK1 silencing in AVS cells transduced with lentiviral vectors carrying different shRNAs (pLVTHMshMEK1-1/-2); negative control pLVTHMshLuciferase. Alpha-tubulin was used as loading control. (F) Rabbit skin infected with pLAII-CRPVshRNAs directed against MEK1 and with pLAII-CRPVshLuciferase. Six skin sites were infected with each construct per rabbit. The injection sites are marked with tattoo ink. Images were taken 6 months post infection.