Fig 5. Functional motifs of MVM NS1N.
(a) Functional motifs mapped onto the MVM NS1N structure. Nuclear localization signal (NLS) (residues 194–195 and 214–216), blue. Acetylation site (Lys85), cyan. Predicted cyclin-dependent kinase (CDK) phosphorylation site (residues 219–223), red. The potential Akt/PKB phosphorylation site at residue Thr278 is located in a region immediately after the C-terminus of the nickase domain (dashed curve). The nickase active site residues are shown in magenta. The putative Ori-binding site (loop L10) overlaps with the NLS and is partially in gold. The N- and C- termini are indicated.
(b) A crystallographic dimer of MVM NS1N view down the 2-fold axis showing that the first element in the NLS, Lys194-195, is adjacent to the second element (Lys214-216) in the NLS from a two-fold-related molecule.