Figure 1.

γ-T-enriched mixed tocopherol diet suppresses tumorigenesis in TRAMP mice. (a) Representative photomicrographs (×100 magnification) of H&E stained dorso-lateral section of non-transgenic, control and treated mice at 24 weeks of age. The genitourinary apparatus was removed en bloc. The dorsolateral prostate was microdissected and analyzed for PIN evaluation. (b) Percentage incidence of normal tissue, low grade and high grade PIN displayed by non-transgenic, control and γ-T-enriched mixed tocopherol treated 24-week old mice. Normal tissue was characterized by single-layered epithelial cells and a distinct lumen, low grade PIN typically displayed a couple of layers of atypical cells that did not, however, fill up the luminal space and high grade PIN characterized by marked stromal thickening and hypercellularity and significantly reduced luminal space. The bars represent mean values ± S.E. Statistical significance of the difference was analyzed by Chi's square test. p values < 0.05 were considered significant. [Color figure can be viewed in the online issue, which is available at www.interscience.wiley.com.]