Figure 3.

γ-T-enriched mixed tocopherol diet increases expression of phase II detoxifying enzymes. (a) Western blot analysis of UGT1A1 and GSTM1 in 24-week old non-transgenic, 8 week and 24-week old TRAMP control and γ-T-enriched mixed tocopherol diet treated samples. (b) Immunohistochemical analysis of GSTM1 in control and γ-T-enriched mixed tocopherol diet treated 24-week old mice. Bars represent mean ± S.E. of relative total score of GSTM1 positively stained cells in control and treated 24-week old mice. No statistically significant difference was observed between control and treated samples. (c) Catalytic activity of GST in non-transgenic, control and treated 24-week old mice. GST activity was determined using 1-chloro-2,4-dinitrobenzene as a substrate. The reaction product, GSH-DNB conjugate absorbs at 340 nm. The rate of increase in absorption is directly proportional to the GST activity in the sample. Bars represent mean values. Student's t test was used to evaluate significance. *Significantly different compared to non-transgenic. ‡Significantly different compared to control. [Color figure can be viewed in the online issue, which is available at www.interscience.wiley.com.]