FIG. 3.

Mixed tocotrienols inhibit tumorigenesis in TRAMP mice. A: Representative photomicrographs (×100 magnification) of H&E stained dorso-lateral section of nontransgenic, control, and mixed tocotrienol fed mice at 24 wk of age. B: Percentage levels of normal tissue, low-grade, and high-grade PIN displayed by nontransgenic, control, and mixed tocotrienol fed 24–wk-old mice. At the time of necropsy, the genitourinary apparatus was removed en bloc. The dorso-lateral prostate was microdissected and analyzed for PIN evaluation. Normal tissue was characterized by single-layered epithelial cells, and a distinct lumen, low-grade PIN typically displayed a couple of layers of atypical cells that did not, however, fill up the luminal space and high-grade PIN characterized by marked stromal thickening and hypercellularity and significantly reduced luminal space. The bars represent mean values ± SE. Statistical significance of the difference between control and treated groups was analyzed by Student’s t-test. P values <0.05 were considered significant.