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. 2016 Jan 4;11(1):e0144561. doi: 10.1371/journal.pone.0144561

Fig 4. Effects of the sGC-cGMP pathway on cell differentiation of murine and human BM progenitors.

Fig 4

(A) Effects of 8-bromo-cGMP and ODQ on the differentiation of murine BM progenitors prepared from sGC transgenic mice and Tg(-) littermates. BMMNC (4 × 104 cells) were analyzed by semi-solid colony assays as described in S1 Text. Results are shown as total numbers of erythroid colonies (red column) and myeloid colonies (blue column). Origin of cells and treatment are as follows: lane 1, Tg(-) (control culture); lane 2, Tg(-) with 1 μM 8-bromo-cGMP; lane 3, Tg(-) with 10 μM 8-bromo-cGMP; lane 4, Tg(-); lane 5, sGC-5; lane 6, sGC-7; lane 7, sGC-5 with 3 μM ODQ; lane 8, sGC-7 with 3 μM ODQ. P values: *, P<0.05; **, P<0.01 compared with control culture. (B) Effects of 8-bromo-cGMP on the differentiation of human BM progenitors. Human BMMNC (4 × 105 cells) were used for semi-solid colony assays as described in Materials and Methods and S1 Text. Results are shown as total number of erythroid colonies (red column) and myeloid colonies (blue column). Lanes: 1, no addition (control culture); 2, 0.01 μM 8-bromo-cGMP; 3, 0.1 μM 8-bromo-cGMP; 4, 1 μM 8-bromo-cGMP; 5, 10 μM 8-bromo-cGMP. P value: *, P<0.05 compared with control culture.