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. 2016 Jan 4;11(1):e0144561. doi: 10.1371/journal.pone.0144561

Fig 5. Effect of the sGC-cGMP pathway on expression of lineage cell-specific transcription factors in murine and human BM progenitors.

Fig 5

(A to E) Altered expression of erythroid- and myeloid cell-specific transcription factors in sGC transgenic mice. Transcription factor expression was determined by RT-PCR as described in Materials & Methods using spleen-derived erythroid cells and peripheral blood leukocytes from sGC mice (sGC-5 and sGC-7, n = 4) and Tg(-) littermates (n = 5). Expression level of transcription factors in Tg(-) littermates (n = 4) was set to 1. P values: *, P<0.05; **, P<0.01 compared with Tg(-) littermates. (F to J) Effect of 8-bromo-cGMP on expression of erythroid- and myeloid cell-specific transcription factors in human BMMNC. Human BM cells (4 × 105 cells) were mixed with Methocult in the presence or absence of various concentrations of 8-bromo-cGMP (0.01 to 20 μM). Cells were isolated from semi-solid colony plates and total RNAs were purified. Expression levels of transcription factors were determined by real time-PCR. P values: *, P<0.05; **, P<0.01 compared with control cultures.