Figure 2. Wounding response after syringe and vacuum infiltration.

Leaf discs from 5 weeks old Arabidopsis plants were either syringe or vacuum infiltrated with 10 mM MgCl₂. Control samples were frozen in liquid nitrogen and left to thaw at room temperature. Leaf discs were analyzed for glycerolipid-bound OPDA at indicated times (A). Free OPDA and JA released by plants are shown in (B) and (C), respectively. (D) Shows dead cells in leaf disc after trypan blue staining. Error bars represent standard deviation from replicate samples.