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. 2016 Jan 4;11(1):e0145998. doi: 10.1371/journal.pone.0145998

Fig 5. Positive feedback for translation of rotavirus mRNA encoding NSP3.

Fig 5

A: schematic representation of the "T2A reporter" mRNA encoding firefly luciferase and wild type or mutated NSP3 ending or not with the GACC sequence. B: BSRT7 cells or C; MA104 cells were electroporated with the capped "T2A reporter" mRNA and a standard Renilla RNA. The Renilla and firefly luciferase activities were measured from .5 to 8 hours after electroporation. The firefly to Renilla (F/R) luciferase ratio is reported. The data are the mean ± SEM of five independent experiments.