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. 2016 Jan 4;11(1):e0145998. doi: 10.1371/journal.pone.0145998

Fig 6. The importance of the 5'UTR length in NSP3- and poly(A)-dependent translation.

Fig 6

BSRT7 cells expressing NSP3 (NSP3; black bars) or no NSP3 (NSP3-KO) (wo. NSP3; white bars) were electroporated with the indicated capped R-RNA molecule (lines 1–6) or capped -p(A)-RNA molecule (lines 7–10) and the standard RNA. The Renilla and firefly luciferase activities were measured 6 hours after electroporation. The Renilla to firefly luciferase ratio is relative to the controls (lines 1 and 7), which was considered 100. The nucleotide sequence upstream the AUG initiation codon is indicated and the 3’UTR is from gene 11. The data are the mean ± SEM of three independent experiments in triplicate. A Student’s t-test was used (*p<0.05;**p<0,01).