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. 2016 Jan 4;11(1):e0145998. doi: 10.1371/journal.pone.0145998

Fig 7. The importance of the initiation codon context in NSP3-dependent translation.

Fig 7

BSRT7 cells expressing NSP3 (black bars) or no NSP3 (NSP3-KO) (wo. NSP3 white bar) were electroporated with the standard RNA and with capped R-RNA (lines 1–6) with 9- (lines 1–3,6) or 5- (lines 4,5) nt-long 5’UTRs with different AUG (bold and italicized) contexts. The -3 and +4 positions are boxed, and the nucleotides that differ from the wild type (line1) are underlined. The Renilla and firefly luciferase activities were measured 6 hours after electroporation. The ratio of Renilla to firefly luciferase activities is reported relative to the control (line 1), which was considered 100. The data are the mean ± SEM of three independent experiments in triplicate. A Student’s t-test was used (*p<0.05;**p<0,01).