Figure 5.

Simultaneous detection of sodium or calcium changes with two different indicators. a, Pyramidal neuron filled with both ANG-2 and SBFI from a patch electrode on the soma. The ANG-2 filter set (Fig. 1d ) was used with excitation filters centered at 380 and 520 nm. A single AP was evoked with a 2 ms intrasomatic current pulse. ANG-2 and SBFI fluorescence changes in the axon were detected in the two channels. The downward slope indicates strong bleaching due to the high light intensity. The combined trace shows the SBFI trace (1.5×) subtracted from the ANG-2 trace. Most of the bleaching is cancelled and the signal enhanced. Typical result from three cells. b, Similar recordings in the dendrite when two calcium indicators were used. The OGB-1 filter set (Fig. 1b ) was used with excitatory filters centered at 387 and 470 nm. The combined trace is bis-fura-2 (BF-2; 1.3×) subtracted from the OGB-1 trace. Typical result from four cells.