Table 1. Identification of peptides released from in gel trypsin digestion of venom protein spots shown in Fig 7 from pooled adult hybrid snakes (H), the mother (M, B. erythromelas), and the father (F, B. neuwiedi), by LC-MS/MS and database search.
| Spot # | Identified peptides * | UniProt entry | Identified Protein,Class ** |
|---|---|---|---|
| H1 | LGVHSIK | VSP1_BOTJA | Platelet-aggregating proteinase PA- |
| INILDHAVCR | BJ, SVSP | ||
| F1 | INILDHAVCR | DISJ_BOTJA | Venom metalloproteinase jararhagin |
| MYELANIVNEIFR | precursor, SVMP | ||
| H6 | CCFVHDCCYGK | PA21B_BOTJA | |
| QICECDRVAATCFR | |||
| M6 | VAATCFR | PA21B_BOTJA | Bothrops jararaca PLA2 (BJ-PLA2), |
| VAATCFRDNK | PLA2 | ||
| CCFVHDCCYGK | |||
| QICECDRVAATCFR | |||
| H7 | VFNEPQNWADAEK | ABA4_TRIAB | Alboaggregin-A subunit 4, CTL |
| ITVKPDVDYTLNSFAEWR | DISJ_BOTJA | Venom metalloproteinase jararhagin | |
| precursor, SVMP | |||
| H12 | MYELANIVNEIFR | DISJ_BOTJA | Venom metalloproteinase jararhagin |
| ITVKPDVDYTLNSFAEWR | precursor, SVMP | ||
| F12 | SVGIVR | DIST_TRIJE | Zinc metalloproteinase jerdonitin |
| precursor, SVMP | |||
| M12 | -.SLVQFETLIMK | PA2_BOTPC | Phospholipase A2, PLA2 |
| M13 | -.SLVQFETLIMK | PA2_BOTPC | Phospholipase A2, PLA2 |
| H14 | -.VVGGDECNINEHR | VSP1_AGKBI | Ancrod, SVSP |
| M14 | WDKDIMLIR | VSP2_BOTJA | Kinin-releasing and fibrinogen- |
| FFCLSSK | clotting serine proteinase 2 | ||
| precursor, SVSP | |||
| M15 | -.VVGGDECNINEHR | VSP1_AGKBI | Ancrod, SVSP |
| H25 | ETVLLNR | DISB_AGKCO | Zinc metalloproteinase acostatin- |
| YNSNLNTIR | beta precursor, SVMP | ||
| F25 | ERDLLPR | BAP1_BOTAS | Hemorrhagic metalloproteinase |
| YNSNLNTIR | BaP1, SVMP | ||
| SVDVHAPLANLEVWSK | |||
| M25 | TLTSFGEWR | LEUCA_BOTLC | Leucurolysin-a, SVMP |
| H26 | HNPQCILNKP | LEUCA_BOTLC | Leucurolysin-a, SVMP |
| F26 | TLTSFGEWR | LEUCA_BOTLC | Leucurolysin-a, SVMP |
| M26 | ETDLLNR | HRTD_CROAT | Hemorrhagic metalloproteinase HT- |
| D/HT-C precursor, SVMP | |||
| H29 | GMVLPGTK | DISJ_BOTJA | Venom metalloproteinase jararhagin |
| NNGDLDKIK | precursor, SVMP | ||
| GNYYGYCR | |||
| KIPCAPEDVK | |||
| F29 | NNGDLDKIK | DISJ_BOTJA | Venom metalloproteinase jararhagin |
| IPCAPEDVK | precursor, SVMP | ||
| AGGRVK | OXLA_BOTMO | L-amino-acid oxidase precursor, | |
| LAAO | |||
| M29 | DLIKVEK | LEUCA_BOTLC | Leucurolysin-a, SVMP |
| ERDLLPR | |||
| YNSNLNTIR | |||
| TLTSFGEWR | |||
| H30 | YFYCR | HR1B_TRIFL | Hemorrhagic metalloproteinase |
| HR1b precursor, SVMP | |||
| F30 | GDEYFYCR | VMSB_TRIST | Zinc metalloproteinase-disintegrin |
| stejnihagin-B, SVMP |
*According to UniProt database. Underlined residues mean carbamidomethylation of cysteines; (-) means N-terminal peptides (for details regarding protein identification see Materials and Methods);
**Toxin class: CTL, C-type lectin; SVMP, snake venom metalloproteinase; LAAO, L-amino acid oxidase; PLA2, phospholipase A2; SVSP, snake venom serine proteinase).